ZnO nanoparticles inhibit the activity of Porphyromonas gingivalis and Actinomycesnaeslundii and promote the mineralization of the cementum. Zinc oxide nanoparticles (ZnONPs) have been widely studied as bactericidal reagents. However, it is still challenging to use ZnONPs as a root canal sealant to eliminate infecting microorganisms in the root canal system. This study aimed at understanding performed to examine the antibacterial activity of ZnONPs against Porphyromonas gingivalis (P. gingivalis) and ActinomycesNaeslundii (A. naeslundii) bacteria in vitro. ZnONPs were further evaluated for their biocompatibility using normal mouse NIH3T3 and OCCM-30 cells by the cell-based MTT assay. In addition, the influence of ZnONPs on matrix metalloproteinases in NIH3T3 cells and their inhibiting
Consumption of apple-boysenberry beverage decreases salivary Actinomycesnaeslundii and their adhesion in a multi-species biofilm model. We hypothesised that consumption of beverage rich in both fibre and polyphenols, rather than each bioactive alone, will modulate populations of selected salivary bacteria, and their adhesion characteristics and that some of these effects may be due to the anti -microbial activity of the beverage bioactives. We investigated the effect of 4 weeks' consumption of beverages, rich in apple fibre, boysenberry polyphenols, or both on salivary bacteria in healthy subjects. In this placebo-controlled crossover study, saliva samples were collected at the beginning and end of each treatment period, and used for qPCR quantitation of Lactobacillus spp., Actinomyces
Genome Sequence of Actinomycesnaeslundii Strain ATCC 27039, Isolated from an Abdominal Wound Abscess Here, we present the complete genome sequence of Actinomycesnaeslundii strain ATCC 27039, isolated from an abdominal wound abscess. This strain is genetically transformable and will thus provide valuable information related to its crucial role in oral multispecies biofilm development.
Antimicrobial Effects of Novel Triple Antibiotic Paste-Mimic Scaffolds on Actinomycesnaeslundii Biofilm. Actinomycesnaeslundii has been recovered from traumatized permanent teeth diagnosed with necrotic pulps. In this work, a triple antibiotic paste (TAP)-mimic scaffold is proposed as a drug-delivery strategy to eliminate A. naeslundii dentin biofilm. Metronidazole, ciprofloxacin
Actinomycesnaeslundii: An Uncommon Cause of Endocarditis Actinomyces rarely causes endocarditis with 25 well-described cases reported in the literature in the past 75 years. We present a case of prosthetic valve endocarditis (PVE) caused by Actinomycesnaeslundii. To our knowledge, this is the first report in the literature of endocarditis due to this organism and the second report of PVE caused
Low-grade-infection after a total knee arthroplasty caused by Actinomycesnaeslundii. Here, we present a case of an 85-year-old woman with a low-grade-infection caused by Actinomycesnaeslundii after total-knee arthroplasty (TKA) followed by septic loosening. Actinomycesnaeslundii was cultured from a tissue sample from the knee joint capsule/synovial tissue obtained after the initial TKA . A review of the literature revealed two cases of periprosthetic infection and another three cases of arthritis due to Actinomycesnaeslundii. So far, no standard treatment for periprosthetic infections caused by Actinomyces species has been established.
-occurrence network tended to be less complex posttreatment. Caries treatment increased the relative abundance of Corynebacterium matruchotii, Corynebacterium durum, Actinomycesnaeslundii, and Saccharibacteria HMT-347, as well as Aggregatibacter HMT-458 and Haemophilus influenzae. Meanwhile, the relative abundance of Streptococcus mutans, three species from Leptotrichia, Neisseria bacilliformis
-tyrosine, and D-tryptophan) that would significantly decrease Enterococcus faecalis and Actinomycesnaeslundii biofilm biomass was first determined. Then, the effect of TC + selected DAAs on polymicrobial biofilms was characterized by quantifying the biomass and biofilm viability. Finally, the antibiofilm effects of TC+DAA was compared with CH and untreated controls by (i) determining bacterial viability
from apical papilla (SCAPs). Antibacterial efficacy was tested using direct and indirect contact assays against Aggregatibacter actinomycetemcomitans/Aa, Actinomycesnaeslundii/An, Enterococcus faecalis/Ef, and Fusobacterium nucleatum/Fn. E. faecalis biofilm inhibition on dentin discs was specifically evaluated for BiMix-laden scaffolds. Data were statistically analyzed with a significance level of 5
. and Actinomycesnaeslundii (A. naeslundii) in the saliva of nursing home elderly, to assess the correlation between bacterial composition and RC for five putative catiogenic organisms. In this study, we collected 43 saliva samples and divided into two groups: the root caries group (RCG, n = 21) and the caries-free group (CFG, n = 22). Bacterial DNA was extracted from the saliva samples. The presence
infected root canals under dynamic loading (simulated chewing). The study was completed in two experiments performed at two institutions. Biofilms of Streptococcus intermedius in the first experiment, and S. intermedius and Actinomycesnaeslundii were allowed to grow in root canals of single-rooted extracted teeth for three weeks. The roots of the teeth were suspended in a small chamber containing dental
received deionized water as a negative control. The specimens were subjected to four cycles (15 h demineralization and 8 h remineralization for one cycle) of biochemical cycling. A mixed suspension of five bacteria species (Streptococcus mutans, Streptococcus sobrinus, Lactobacillus acidophilus, Lactobacillus rhamnosus, and Actinomycesnaeslundii) were prepared in brain heart infusion broth with 5
hyclate gel, respectively. The clinical assessments of Gingival Index (GI), Plaque Index (PI), Probing Pocket Depth (PPD), and Clinical Attachment Level (CAL) together with microbial colony counts for Porphyromonas gingivalis, Actinomycesnaeslundii, and Prevotella intermedia were done at baseline, 1st month, and 3rd month follow-ups. Results: The results showed there was a significant reduction
teeth. The specimens were exposed to citric acid (pH 3.2) for 30 minutes and randomly divided into three groups (n = 15): Group 1, no biofilm; Group 2, 1-day-old biofilm; and Group 3, 3-day-old biofilm. Specimens in Groups 2 and 3 were inoculated with oral bacteria (Streptococcus sanguinis, Streptococcus mitis, and Actinomycesnaeslundii) to produce early laboratory-cultivated biofilms for 1
wine and oenological extracts, rich in polyphenols, against the periodontal pathogens Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum and total bacteria growing in an in vitro oral biofilm static model. A previously validated biofilm model, including Streptococcus oralis, Actinomycesnaeslundii, Veillonella parvula, F. nucleatum, P. gingivalis
Curcumin-A Natural Medicament for Root Canal Disinfection: Effects of Irrigation, Drug Release, and Photoactivation. Curcumin incorporation into polymeric fibers was tested for its antimicrobial properties and potential use in root canal disinfection. Curcumin-modified fibers were processed via electrospinning and tested against a 7-day old established Actinomycesnaeslundii biofilm
bacterial biofilm formation. Highly pure and structurally intact Lp.LTA was purified from L. plantarum. Actinomycesnaeslundii, Lactobacillus salivarius, Streptococcus mutans, and Enterococcus faecalis were co-cultured to form oral multispecies biofilm in the presence or absence of Lp.LTA on culture plates or human dentin slices. Preformed biofilm was treated with or without Lp.LTA, followed by additional
of Actinomycesnaeslundii and lower level of relative abundance of a species belonging to Candidate_division_TM7 was observed in dental plaque of EBS-positive subjects, compared to dental plaque of EBS-free subjects (P < 0.05). This indicated that some species might be involved in the EBS process. Changes in dental plaque microbiota is possibly relevant to the process of EBS in the primary dentition.
biofilm development. A reproducible and easy-to-use biofilm model as a test system for large scale screening of new implant surfaces with putative antibacterial potency is therefore of major importance. In the present study, we developed a highly reproducible in vitro four-species biofilm model consisting of the highly relevant oral bacterial species Streptococcus oralis, Actinomycesnaeslundii
in 18 adult volunteers. The color reaction was read after 1 h in room temperature and scored as in the in vitro test. The strongest activity was registered for Staphylococcus epidermidis, Helicobacter pylori, Campylobacter ureolyticus and some strains of Haemophilus parainfluenzae, while known ureolytic species such as Streptococcus salivarius and Actinomycesnaeslundii showed a weaker, variable