Allogeneic limbo-deep anterior lamellar keratoplasty (Limbo-DALK)-A novel surgical technique in corneal stromal disease and limbalstemcell deficiency. To describe a novel corneal surgical technique combining Deep Anterior Lamellar Keratoplasty (DALK) with grafting of allogeneic limbus (Limbo-DALK) for the treatment of eyes with corneal stromal pathology and limbalstemcell deficiency (LSCD
Ex vivo expanded autologous human corneal epithelial cells containing stem cells (Holoclar) - For the treatment of adult patients with moderate to severe limbalstemcell deficiency (defined by the presence of superficial corneal neovascularisation in at 1 Published 7 September 2020 1 SMC2261 ex vivo expanded autologous human corneal epithelial cells containing stem cells (Holoclar® human corneal epithelial cells containing stem cells (Holoclar®) is accepted for use within NHSScotland on an interim basis subject to ongoing evaluation and future reassessment. Indication under review: Treatment of adult patients with moderate to severe limbalstemcell deficiency (defined by the presence of superficial corneal neovascularisation in at least two corneal quadrants, with central
A novel therapy to ameliorate nitrogen mustard-induced limbalstemcell deficiency using lipoprotein-like nanoparticles. Chronic corneal inflammation, a component of sulfur mustard (SM) and nitrogen mustard (NM) injuries frequently leads to limbalstemcell deficiency (LSCD), which can compromise vision. Corneal conjunctivalization, neovascularization, and persistent inflammation are hallmarks
Correlation between anterior chamber angle status and limbalstemcell deficiency in primary angle-closure glaucoma. To investigate the correlation between the opening and closing states of anterior chamber angle (ACA) and the density of limbal epithelial basal cells (LEBCs) in subjects with primary angle-closure glaucoma (PACG). Cross-sectional observational study. A total of 54 eyes of 29 . Advanced PACG and angle closure should be highly suspected of the occurrence of limbalstemcell deficiency (LSCD).
Limbalstemcell therapy. To highlight the progress and future direction of limbalstemcell (LSC) therapies for the treatment of limbalstemcell deficiency (LSCD). Direct LSC transplantation have demonstrated good long-term outcomes. Cultivated limbal epithelial transplantation (CLET) has been an alternative to treat severe to total LSCD aiming to improve the safety and efficacy of the LSC
Outcomes and Complications of LimbalStemCell Allograft Transplantation: A Report by the American Academy of Ophthalmology. To review the published literature on the safety and outcomes of keratolimbal allograft (KLAL) transplantation and living-related conjunctival limbal allograft (lr-CLAL) transplantation for bilateral severe/total limbalstemcell deficiency (LSCD). Literature searches were
Co-existence of Neurotrophic Keratopathy in Eyes with LimbalStemCell Deficiency. To investigate whether neurotrophic keratopathy is present in limbalstemcell deficiency (LSCD) by measuring corneal sensation and characterizing corneal subbasal nerve plexus. Prospective, cross-sectional "case-control" comparative study. Forty-six eyes with LSCD and 14 normal eyes were recruited from 2019
Retracted: Acupoint Massage plus Recombinant Bovine Basic Fibroblast Growth Factor Ophthalmic Gel and LimbalStemCell Transplantation on Visual Quality, Corneal Refraction, and Aesthetic Outcome in Patients with Pterygium. [This retracts the article DOI: 10.1155/2022/7103496.].
In Vivo Confocal Microscopy in LimbalStemCell Deficiency After Mesenchymal Stem Cell Transplantation: A Sub-analysis from a Phase I-II Clinical Trial. The aim of this work is to evaluate the effect of mesenchymal stem cell transplantation (MSCT) and cultivated limbal epithelial transplantation (CLET) therapies on the limbus of patients suffering from limbalstemcell deficiency (LSCD). A sub
Management of limbalstemcell deficiency by amnion-assisted conjunctival epithelial redirection using vacuum-dried amniotic membrane and fibrin glue. To study the outcome of a modified amnion-assisted conjunctival epithelial redirection (ACER) technique using vacuum-dried amnion (Omnigen) and fibrin glue for managing total limbalstemcell deficiency (LSCD). A retrospective, interventional case series of all patients with total LSCD who underwent limbalstemcell transplant (LSCT) using the modified ACER procedure between 2016 and 2019. The outcome was defined as: (1) success: complete corneal re-epithelialisation without conjunctivalisation; (2) partial success: sub-total corneal re-epithelialisation with partial non-progressive conjunctivalisation sparing the visual axis and (3) failure
Clinical Trial of Autologous Cultivated Limbal Epithelial Cell Sheet Transplantation for Patients with LimbalStemCell Deficiency. To confirm the efficacy and safety of Good Manufacturing Practice (GMP)-compliant autologous cultivated limbal epithelial cell sheets in government-controlled clinical trials that adhered to Good Clinical Practice stipulations for patients with unilateral limbalstemcell deficiency (LSCD). A prospective, multicenter, open-label, uncontrolled, single-arm clinical trial. Ten consecutive eyes of 10 patients with unilateral LSCD were followed for 2 years after surgery. Preoperative LSCD stage was IIB in 4 eyes and III in 6 eyes. A limbal tissue biopsy was obtained from the healthy eye, after which limbalstemcells were dissociated and cultivated on temperature
Cultivated Autologous Limbal Epithelial Cell Transplantation: New Frontier in the Treatment of LimbalStemCell Deficiency. Taking into consideration prior human experience with treating limbalstemcell deficiency (LSCD) with cultivated limbal epithelial cells (CLEC) from other countries, we have set a goal to optimize and standardize the techniques of CLEC preparation (called CALEC by our
Acupoint Massage plus Recombinant Bovine Basic Fibroblast Growth Factor Ophthalmic Gel and LimbalStemCell Transplantation on Visual Quality, Corneal Refraction, and Aesthetic Outcome in Patients with Pterygium. To investigate the clinical study of acupoint massage combined with recombinant bovine basic fibroblast growth factor (rbFGF) ophthalmic gel combined with limbalstemcell transplantation on visual quality, corneal refraction, and aesthetic outcome in patients with pterygium. Sixty patients with pterygium treated in People's Hospital of Lujiang County from March 2018 to May 2021 were randomized into the control group and the research group by the random number table method, with 30 cases in each group. The control group received rbFGF plus limbalstemcell transplantation
Immunocytochemistry of the ocular surface after different techniques of limbalstemcell transplantation for chronic chemical burns. To compare the immunocytochemistry (ICC) on impression cytology of corneal surface epithelium after simple limbal epithelial transplantation (SLET) and conjunctival-limbal autograft (CLAU). A prospective study of 20 patients above 1 year of age with chronic chemical burns, who underwent limbalstemcell transplantation (LSCT). They were divided equally in group A (SLET) and group B (CLAU). ICC was done for cytokeratin 3 (CK3) and cytokeratin 19 (CK19), preoperatively and postoperatively at 6 months. Four cases were excluded due to inadequate cellularity in preoperative or postoperative samples. On ICC analysis, in the remaining 16 patients mean CK3 and CK19
Economic, clinical and social impact of simple limbal epithelial transplantation for limbalstemcell deficiency. To report the global uptake of simple limbal epithelial transplantation (SLET) and compare the economic, clinical and social outcomes of SLET with those of cultured limbal epithelial transplantation (CLET). A comprehensive literature review and an online survey of eye surgeons were attractive alternative to CLET to treat limbalstemcell deficiency.
Holoclar for the treatment of limbalstemcell deficiency due to ocular burns Holoclar for the treatment of limbalstemcell deficiency due to ocular burns ..
Limbalstemcell deficiency secondary to systemic paclitaxel (Taxol) for breast cancer: a case report. Paclitaxel (PTX) is an antineoplastic drug widely used in treatments for ovarian, breast, and small-cell lung cancer. Although ocular effects associated with PTX have been previously described, very few studies have specifically reported systemic PTX as a contributing factor for limbalstem , and features of limbalstemcell deficiency (LSCD) were noted on the right cornea. A central neurotrophic ulcer with thinning to 50% and 360 degrees of conjunctivalization were noted on the left. After the discontinuation PTX with doxorubicin as the substitute, there was no further progression of her LSCD, and stabilization of her ocular surface was achieved. Although chemotherapy induced LSCD
Multi-parametric evaluation of autologous cultivated Limbal epithelial cell transplantation outcomes of Limbalstemcell deficiency due to chemical burn. The sparsity of established tools for the grading of limbalstemcell deficiency hinder objective assessments of the clinical outcome of cultivated limbal epithelial cell transplantation. To advance towards the development of standards for the comparison of the outcomes of these bio-surgical protocols we have now applied a battery of recognized objective and patient-declared subjective outcome criteria to the autologous modality of cultivated limbal epithelial cell transplantation. The prospective study involved ten patients (M/F = 9/1; mean age = 42.1 years) displaying overt unilateral limbalstemcell deficiency complying with the inclusion
Epithelial Cell Migration and Proliferation Patterns During Initial Wound Closure in Normal Mice and an Experimental Model of LimbalStemCell Deficiency. Establishing the dynamics of corneal wound healing is of vital importance to better understand corneal inflammation, pathology, and corneal regeneration. Numerous studies have made great strides in investigating multiple aspects of corneal wound healing; however, some aspects remain to be elucidated. This study worked toward establishing (1) if epithelial limbalstemcells (LSCs) are necessary for healing all corneal wounds, (2) the mechanism by which epithelial cells migrate toward the wound, and (3) if centrifugal epithelial cell movement exists. To establish different aspects of corneal epithelial wound healing we subjected mice