"Molecular diagnostics" from_date:2012

IDSA Guidelines on the Diagnosis of COVID-19: Molecular Diagnostic Testing Skip to navSkip to contentLoginView all GuidelinesFacebook Twitter LinkedIn EmailGet the Guidelines App!IDSA Guidelines on the Diagnosis of COVID-19: Molecular Diagnostic TestingPublished by IDSA on 5/6/2020. Last updated, 9/5/2023COVID-19 Guideline, Part 1: Treatment and ManagementCOVID-19 Guideline, Part 2: Infection

The Infectious Diseases Society of America Guidelines on the Diagnosis of COVID-19: Molecular Diagnostic Testing Last updated December 23, 2020 and posted online at www.idsociety.org/COVID19guidelines/dx. Please check website for most updated version of these guidelines. Supplementary materials can be found here. The Infectious Diseases Society of America Guidelines on the Diagnosis of COVID-19 : Molecular Diagnostic Testing Authors Kimberly E. Hanson,1 Angela M. Caliendo,2 Cesar A. Arias,3 Mary K. Hayden,4 Janet A. Englund,5 Mark J. Lee,6 Mark Loeb,7 Robin Patel,8 Abdallah El Alayli,9 Osama Altayar,10 Payal Patel,11 Yngve Falck-Ytter,12 Valery Lavergne,13 Rebecca L. Morgan,14 M. Hassan Murad,15 Shahnaz Sultan,16 Adarsh Bhimraj,17 Reem A. Mustafa18 Affiliations 1Department of Internal Medicine

Guidelines vs mindlines: a qualitative investigation of how clinicians' beliefs influence the application of rapid molecular diagnostics in intensive care. Rapid molecular diagnostic tests improve antimicrobial stewardship (AMS) by facilitating earlier refinement of antimicrobial therapy. The INHALE trial tested the application of the BioFire FilmArray Pneumonia Panel (Pneumonia Panel to patient care.IMPORTANCERapid molecular diagnostic tests for pathogens and resistance genes may improve antibiotic-prescribing decisions and stewardship. However, clinicians' desire to protect their patients with antibiotics often overrides more distal concerns about possible resistance selection, limiting the application of these tests in practice. Findings underscore the challenge of changing

The Microbiology and Clinical Presentation of Acute Bacterial Arthritis in Houston Area Children <5 Years Old in the Era of Molecular Diagnostics. While Staphylococcus aureus is the most common pathogen causing acute bacterial arthritis (ABA), the microbiology is diverse, particularly in young children. Kingella kingae is a well-known pathogen of ABA and can be particularly difficult to identify . We examined the impact of molecular diagnostics on ABA in a historically methicillin-resistant S. aureus (MRSA) endemic region. Cases of ABA in children ≤5 years old between 2015 and 2022 were reviewed. The clinical features of cases were compared by causative pathogen. Trends in utilization of molecular diagnostics and rates of pathogen identification were examined. One hundred sixty-two eligible

Integrating Prenatal Exome Sequencing and Ultrasonographic Fetal Phenotyping for Assessment of Congenital Malformations: High Molecular Diagnostic Yield and Novel Phenotypic Expansions in a Consanguineous Cohort. To evaluate the diagnostic yield of prenatal exome sequencing (pES) in fetuses with structural anomalies detected by prenatal ultrasound in a consanguineous population

Sensitive diagnosis of paucibacillary tuberculosis with targeted next-generation sequencing: a molecular diagnostic study. Targeted next-generation sequencing (tNGS) enables high-performance tuberculosis (TB) diagnosis and drug resistance prediction directly from clinical samples. However, its applicability to paucibacillary TB, including pediatric TB and extrapulmonary TB (EPTB), has been less

Azithromycin for bacterial watery diarrhea: A reanalysis of the AntiBiotics for Children with severe Diarrhea (ABCD) trial incorporating molecular diagnostics Bacterial pathogens cause substantial diarrhea morbidity and mortality among children living in endemic settings, yet antimicrobial treatment is only recommended for dysentery or suspected cholera. AntiBiotics for Children with severe

Effect of mixed Mycobacterium tuberculosis infection on rapid molecular diagnostics among patients starting MDR-TB treatment in Uganda. Mixed M. tuberculosis (MTB) infection occurs when one is infected with more than one clonally distinct MTB strain. This form of infection can assist MTB strains to acquire additional mutations, facilitate the spread of drug-resistant strains, and boost the rate of treatment failure. Hence, the presence of mixed MTB infection could affect the performance of some rapid molecular diagnostic tests such as Line Probe Assay (LPA) and GeneXpert MTB/RIF (Xpert) assays. This was a cross-sectional study that used sputum specimens collected from participants screened for STREAM 2 clinical trial between October 2017 and October 2019. Samples from 62 MTB smear-positive patients

Caveats in Interpretation of Molecular Diagnostics in Heart Allografts. Histologic separation of injury, T cell-mediated rejection, or antibody-mediated rejection in allograft heart biopsies is difficult. A critical review of publications was performed to evaluate the caveats of using molecular diagnostics (MDX) to distinguish between these entities. Typically, only 1 to 2 fragments of unknown

Comparison of transbronchial biopsy techniques using needle and forceps biopsies in lung cancer for molecular diagnostics: a prospective, randomized crossover trial. In lung cancer, molecular testing and next-generation sequencing (NGS) are needed to identify therapeutic targets and are increasingly being used in earlier stages of the disease. Despite its longstanding use, it remains unclear

CRISPR-based rapid molecular diagnostic tests for fusion-driven leukemias. Fusion oncogenes can be cancer-defining molecular alterations that are essential for diagnosis and therapy selection.1,2 Rapid and accessible molecular diagnostics for fusion-driven leukemias such as acute promyelocytic leukemia (APL), Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph+ ALL), and chronic

Identification of a measles variant displaying mutations impacting molecular diagnostics, Geneva, Switzerland, 2023. Real-time PCR is one of the most widely used techniques to diagnose measles cases. Here we report measles virus variants with three genetic mutations in the reverse primer annealing site of a widely used PCR. The mutations result in a slight loss of the PCR sensitivity. Variants

Variant classification changes over time in the clinical molecular diagnostic laboratory setting. Variant classification in the setting of germline genetic testing is necessary for patients and their families to receive proper care. Variants are classified as pathogenic (P), likely pathogenic (LP), uncertain significance (VUS), likely benign (LB) and benign (B) using the standards and guidelines

Detection of Duffy blood group genotypes and submicroscopic Plasmodium infections using molecular diagnostic assays in febrile malaria patients. Malaria remains a severe parasitic disease, posing a significant threat to public health and hindering economic development in sub-Saharan Africa. Ethiopia, a malaria endemic country, is facing a resurgence of the disease with a steadily rising infections. Except for one patient with P. falciparum infection, Plasmodium infections in Duffy-negative individuals were all submicroscopic with low parasitaemia. The present study revealed a high prevalence of submicroscopic malaria infections. Plasmodium vivax infections in Duffy-negative individuals were not detected due to low parasitaemia. In this study, an improved molecular diagnostic tool was used

Racial and socioeconomic disparities in NSCLC molecular diagnostics uptake. Precision therapies, such as targeted and immunotherapies, have substantially changed the landscape of late-stage non-small cell lung cancer (NSCLC). Yet utilization of these therapies is disproportionate across strata defined by race and socioeconomic status (SES), possibly due to disparities in molecular diagnostic testing (or "biomarker testing"), which is a prerequisite to treatment. We extracted a cohort of NSCLC patients from the Surveillance, Epidemiology and End Results (SEER)-Medicare linked data. The primary outcome was receipt of a molecular diagnostic test, based on claims data. The primary predictors were race and SES. Likelihood of receiving a molecular diagnostic test, and overall survival (OS), were

Molecular diagnostic yield of exome sequencing in a Chinese cohort of 512 fetuses with anomalies. Currently, whole exome sequencing has been performed as a helpful complement in the prenatal setting in case of fetal anomalies. However, data on its clinical utility remain limited in practice. Herein, we reported our data of fetal exome sequencing in a cohort of 512 trios to evaluate its

Testing of rapid evaporative mass spectrometry for histological tissue classification and molecular diagnostics in a multi-site study. While REIMS technology has successfully been demonstrated for the histological identification of ex-vivo breast tumor tissues, questions regarding the robustness of the approach and the possibility of tumor molecular diagnostics still remain unanswered

Rapid Molecular Diagnostics in Vulvovaginal Candidosis. Vulvovaginal candidosis (VVC) is a common condition among women, with current diagnostic methods relying on clinical evaluation and laboratory testing. These traditional methods are often limited by the need for specialized training, variable performance, and lengthy diagnostic processes, leading to delayed treatment and inappropriate antifungal use. This review evaluates the efficacy of molecular diagnostic tools for VVC and provides guidance on their application in clinical practice. A literature search was conducted using PubMed to identify studies evaluating rapid diagnostic tests specifically for vulvovaginal isolates. Inclusion criteria focused on studies utilizing molecular diagnostics for the detection of species in VVC

'A comparative study of traditional and molecular diagnostic methods for detection of gastrointestinal parasites in Nepalese migrants to the UK'. We evaluated the results of examining a single faecal sample for gastrointestinal parasites (GIP) using a combination of traditional methods with multiplex qPCR for helminths and protozoa, compared to a reference standard of examining three faecal infections when both were compared to the reference standard. Combining molecular and traditional methods to analyse a single stool improved the detection rate for most studied parasites. This approach has value in settings where repeated sampling and/or faecal culture for helminths is impractical, but molecular diagnostics are available.

The Application of Digital PCR as a Reference Measurement Procedure to Support the Accuracy of Quality Assurance for Infectious Disease Molecular Diagnostic Testing. Nucleic acid amplification tests (NAATs) assist in the diagnosis of numerous infectious diseases. They are typically sensitive and specific and can be quickly developed and adapted. Far more challenging is the development