Clinical Predictors of Micro-TESE Success in Non-Obstructive Azoospermia with Complete AZFc Microdeletion. To evaluate the clinical and histopathological parameters of sperm retrieval success using micro-TESE in NOA with complete AZFc microdeletion, since there is limited data in the literature on the outcomes of this patient group and controversial results on the parameters affecting the success of micro-TESE in non-obstructive azoospermia (NOA) patients. The data of 1,308 patients with NOA who underwent micro-TESE surgery at two centers between 2014 and 2022 were retrospectively analyzed. Clinical and histopathological data were comparatively assessed in men with complete AZFc microdeletion according to sperm retrieval success. Among the 1,308 men, 54 (4.1%) were diagnosed
Unraveling a subgroup of men with unexplained male infertility - men with normogonadotropic non-obstructive azoospermia. Non-obstructive azoospermia (NOA) constitutes male infertility with complete absence of sperm in the ejaculate. NOA can originate in testicular malfunction or in endocrine dysregulation. Elevated FSH levels are diagnostically valuable for NOA. An azoospermic patient cohort
Systematic molecular analyses for 115 karyotypically normal men with isolated non-obstructive azoospermia. Do copy-number variations (CNVs) in the azoospermia factor (AZF) regions and monogenic mutations play a major role in the development of isolated (non-syndromic) non-obstructive azoospermia (NOA) in Japanese men with a normal 46, XY karyotype? Deleterious CNVs in the AZF regions
Sperm retrieval outcomes of contralateral testis in men with nonobstructive azoospermia and unsuccessful unilateral microdissection testicular sperm extraction. ▪▪▪.
Association of DAZ and DAZLA Gene Loci with Spermatogenesis in Patients with Idiopathic Azoospermia and Severe Oligospermia. To explore the relationship between DAZ (Deleted in Azoospermia, DAZ) and DAZLA (Deleted in Azoospermia-like autosomal) gene deletion and male idiopathic azoospermia and oligozoospermia. 80 patients with azoospermia (azoospermia group) and 80 patients with oligozoospermia parameters among the three groups were compared. The azoospermia were stratified according to whether DAZ and DAZLA gene locus deletion occurred. DAZ gene locus deletion rate in azoospermia and oligospermia groups was considerably higher than in the control group (P < .05). The DAZLA gene locus deletion rate in the azoospermia group was apparently higher than that in the oligospermia and control groups (P
Clinical Factors Impacting Microdissection Testicular Sperm Extraction Success in Hypogonadal Men with Nonobstructive Azoospermia. To explore factors influencing microdissection testicular sperm extraction (micro-TESE) success in hypogonadal men with nonobstructive azoospermia (NOA). Cohort study. University-affiliated male reproductive health center. 616 consecutive NOA patients
Whole genome sequencing identifies a homozygous splicing variant in TDRKH segregating with non-obstructive azoospermia in an Iranian family. Non-obstructive azoospermia (NOA) resulting from primary spermatogenic failure represents one of the most severe forms of male infertility, largely because therapeutic options are very limited. Beyond their diagnostic value, genetic tests for NOA also hold
A nomogram predicting testicular sperm extraction success in men with non-obstructive azoospermia: A multi-center study. To develop an efficient and easy-to-use nomogram which can predict testicular sperm extraction(TESE) success in men with non-obstructive azoospermia(NOA) by using pre-operative parameters. Data of 3093 men who underwent TESE for NOA were included in this study. Demographic
Clinical effectiveness of unilateral single-armed vasoepididymostomy in obstructive azoospermia: a single-center experience. Vasoepididymostomy (VE) is an important surgical treatment to achieve natural conception for patients with obstructive azoospermia (OA), and only unilateral VE can be performed under certain conditions, such as OA patients with congenital unilateral absence of the vas
Microdissection Testicular Sperm Extraction Versus Multiple Needle-pass Percutaneous Testicular Sperm Aspiration in Men with Nonobstructive Azoospermia: A Randomized Clinical Trial Surgical extraction of testicular spermatozoa is needed in men with nonobstructive azoospermia (NOA) who wish to become biological fathers. Based on available uncontrolled studies with unspecific patient selection comparison, SRR was higher in mTESE than in multiple needle-pass TESA. Men with azoospermia need surgical extraction of spermatozoa to become biological fathers. In this randomized trial, we compared two surgeries (microdissection testicular sperm extraction [mTESE] and testicular sperm aspiration [TESA]) and found that mTESE gives a higher sperm retrieval rate than multiple needle-pass TESA.
Unilateral Versus Bilateral Vasoepididymal Anastomosis for Idiopathic Obstructive Azoospermia: A Randomised Controlled Trial. Men with idiopathic obstructive azoospermia (OA) are candidates for surgical reconstruction with a vasoepididymal anastomosis (VEA) performed on one or both testis. There are no randomised trials comparing the success of unilateral versus bilateral VEA. We conducted
Genetic Architecture of Azoospermia-Time to Advance the Standard of Care. Crypto- and azoospermia (very few/no sperm in the semen) are main contributors to male factor infertility. Genetic causes for spermatogenic failure (SPGF) include Klinefelter syndrome and Y-chromosomal azoospermia factor microdeletions, and CFTR mutations for obstructive azoospermia (OA). However, the majority of cases remain unexplained because monogenic causes are not analysed. To elucidate the monogenic contribution to azoospermia by prospective exome sequencing and strict application of recent clinical guidelines. Since January 2017, we studied crypto- and azoospermic men without chromosomal aberrations and Y-chromosomal microdeletions attending the Centre of Reproductive Medicine and Andrology, Münster. We
Azoospermia and Sperm Retrieval in Post-Pubertal Testicular Torsion; Benefits and limitations. To assess the risk of azoospermia development and the value of sperm retrieval in post-pubertal testicular torsion (TT). This prospective study included patients with post-pubertal TT. Surgical exploration was urgently performed with either orchiopexy or orchiedectomy and contralateral orchiopexy . With the intention of cryopreservation, all cases underwent conventional testicular sperm extraction. Patients were followed-up after 1, 3, and 6 months with semen analysis and hormonal assay (FSH, LH and testosterone). Sperm retrieval rate (SRR), azoospermia rate and changes in hormonal profile were evaluated. The study included 62 patients with a median (IQR) age of 19 (18-20.7) years and duration of testicular
A homozygous frameshift variant in SYCP2 caused meiotic arrest and non-obstructive azoospermia. Genetic causation for the majority of non-obstructive azoospermia (NOA) remains unclear. Mutations in synaptonemal complex (SC)-associated genes could cause meiotic arrest and NOA. Previous studies showed that heterozygous truncating variants in SYCP2 encoding a protein essential for SC formation , are associated with non-obstructive azoospermia and severe oligozoospermia. Herein, we showed a homozygous loss-of-function variant in SYCP2 (c.2689_2690insT) in an NOA-affected patient. And this variant was inherited from heterozygous parental carriers by natural reproduction. HE, IF, and meiotic chromosomal spread analyses demonstrated that spermatogenesis was arrested at the zygotene stage in the proband
Molecular mechanisms of cellular dysfunction in testes from men with non-obstructive azoospermia. Male factor infertility affects 50% of infertile couples worldwide; the most severe form, non-obstructive azoospermia (NOA), affects 10-15% of infertile males. Treatment for individuals with NOA is limited to microsurgical sperm extraction paired with in vitro fertilization intracytoplasmic sperm
A bi-allelic REC114 loss-of-function variant causes meiotic arrest and nonobstructive azoospermia. Nonobstructive azoospermia (NOA), the most severe manifestation of male infertility, lacks a comprehensive understanding of its genetic etiology. Here, a bi-allelic loss-of-function variant in REC114 (c.568C > T: p.Gln190*) were identified through whole exome sequencing (WES) in a Chinese NOA
Effect of maca (Lepidium meyenii) extract on non-obstructive azoospermia in male mice. Maca (Lepidium meyenii) is a medicinal and edible plant that has a long history attention because of its potential to improve fertility and sexual function. In this study, effects of maca on azoospermia were investigated. The effective components of maca were screened. The therapeutic action of maca were , oligosaccharides and small molecules fractions for preliminary screening of efficacy. These results showed that there was no significant difference between the maca polysaccharide fraction and maca extract. The results were shown that maca can improve non-obstructive azoospermia, the polysaccharide fraction was the active components.
Novel MEIOB pathogenic variants including a homozygous non-canonical splicing variant, cause meiotic arrest and human non-obstructive azoospermia. Non-obstructive azoospermia (NOA) is the most severe form of human male infertility, and the genetic causes of NOA with meiotic arrest remain largely unclear. In this study, we identified novel compound heterozygous MEIOB variants (c.814C > T: p.R272X
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