"Complement fixation test"

Evaluation of the comparative accuracy of the complement fixation test, Western blot and five enzyme-linked immunosorbent assays for serodiagnosis of glanders. Glanders is a zoonotic contagious disease of equids caused by Burkholderia (B.) mallei. Serodiagnosis of the disease is challenging because of false-positive and false-negative test results. The accuracy of the complement fixation test

Bayesian Validation of the Indirect Immunofluorescence Assay and Its Superiority to the Enzyme-Linked Immunosorbent Assay and the Complement Fixation Test for Detecting Antibodies against Coxiella burnetii in Goat Serum Although many studies have reported the indirect immunofluorescence assay (IFA) to be more sensitive in detection of antibodies to Coxiella burnetii than the complement fixation test (CFT), the diagnostic sensitivity (DSe) and diagnostic specificity (DSp) of the assay have not been previously established for use in ruminants. This study aimed to validate the IFA by describing the optimization, selection of cutoff titers, repeatability, and reliability as well as the DSe and DSp of the assay. Bayesian latent class analysis was used to estimate diagnostic specifications

Development of a Sterne-Based Complement Fixation Test to Monitor the Humoral Response Induced by Anthrax Vaccines Anthrax is a zoonotic disease caused by Bacillus anthracis spore-forming bacterium. Since it is primarily a disease of animals, the control in animals, and humans depend on the prevention in livestock, principally cattle, sheep, and goats. Most veterinary vaccines utilize for an effective anthrax surveillance. In this study, we developed a Sterne-based Complement Fixation Test (CFT) to detect specific antibodies induced in animals vaccinated with Sterne 34F2. We assessed its efficacy in laboratory animals and under field conditions by monitoring the humoral response induced by vaccination in cattle. The results indicated that the Sterne-based CFT is able to correctly identify

Complement Fixation Test: An Update of an Old Method for Diagnosis of Bullous Pemphigoid. The complement fixation test (CFT) is a method traditionally used for diagnosis of gestational pemphigoid. Its performance in diagnosis of bullous pemphigoid (BP) has not been investigated in a large patient cohort. The aim of this single-centre, retrospective, serological case-control study of 300 patients

factorsLog in or subscribe to access all of BMJ Best PracticeDiagnostic investigations1st investigations to order * CT and/or MRI head scan * fungal blood cultures (3 sets) * serum cryptococcal antigen test * serum + urine Histoplasma antigen * immunodiffusion tests (IgM and IgG) and complement fixation test (IgG) for coccidioidomycosis * cerebrospinal fluid opening pressure * cerebrospinal fluid (CSF) WBC

in sera from cattle. The developed ciELISA was validated using 2833 serum samples from dairy and beef cattle. From these, 1515 sera were from uninfected cows that belonged to free of brucellosis herds and 1318 were from infected cows that belonged positive to brucellosis herds. Sera were analyzed with the developed ciELISA, the buffer plate antigen (BPA) test, and the complement fixation test (CFT

animal products (90.0%). Accordingly, 149 blood samples from animal owners and 17 farmworkers were collected for serological testing. The serum samples collected were initially screened using the Rose Bengal Plate test, and the Complement Fixation test was used as a confirmatory test. The overall seroprevalence of zoonotic brucellosis was 1.2% (95%CI: 0.32-4.27). There was a statistically significant

mass index BNP B-type natriuretic peptide C3 Complement component C3 C4 Complement component C4 CA15.3 Carbohydrate antigen 15.3 CA19.9 Carbohydrate antigen 19.9 CCP Cyclic citrullinated peptide antibody CEA Carcinoembryonic antigen CFA Coagulation factor assay CFT Complement fixation test CG Clinical Guideline CKD Chronic kidney disease CRP C-reactive protein CS Clotting screen CSF Cerebrospinal

(BALF) through metagenomic next-generation sequencing (mNGS). C. pecorum infection was further identified by qPCR and complement fixation test. The patient improved rapidly after targeted treatment. He was a farmer with diabetes mellitus, and had a history of close contact with sheep, which might result in C. pecorum infection. Our report could provide a direction for the diagnosis and treatment

Blood tests There may be raised WCC or (rarely) evidence of haemolytic anaemia. ESR may be elevated. Serology titres and complement fixation tests/ELISA can help to confirm the diagnosis. FBC may show left shift. Severe cases may have DIC evident on FBC/INR. Hyponatraemia may occur due to syndrome

or ocular cysts See Presentation for more detail.Diagnosis of cysticercosisLaboratory studiesThe enzyme immunotransfer blot assay for the detection of serum and cerebrospinal fluid (CSF) antibodies to T solium is the antibody test of choice for cysticercosis.The complement fixation test for the detection of antibodies in CSF is highly specific and sensitive.Imaging studiesThe diagnosis

with the peptide-based ELISA (1/84; 1.2%) in Australian sheep yet moderate seropositivity in a Swiss flock with history of C. abortus-associated abortions (17/63; 26.9%). By whole cell antigen complement fixation tests (CFT) and ELISA, chlamydial seropositivity was significantly higher in all groups, suggesting cross-reactivity between these two chlamydial species and non-specificity of the tests. However, only

-effective and accurate method, and confirmation of the true positive reactors by the complement fixation test, a highly specific method that is less sensitive to vaccine-induced antibodies. We demonstrate how, despite the high vaccination coverage of the small ruminant population in Israel, our results proved to be effective in discriminating between vaccinated and infected animals. The speed and accuracy

zones. Using multistage sampling, serum samples of 2409 sheep from 274 flocks were collected and tested using the Rose Bengal Plate Agglutination Test (RBPT) and positive sera were confirmed using a Complement Fixation Test (CFT). Of all animals tested, 4.98% were RBPT positive, and after confirmation with CFT, the overall animal-level prevalence was found to be 4.89% (CI: 3.24-6.9%). Of the flocks

acidified plate antigen test and rose Bengal test as screening tests and complement fixation test as a confirmatory test. Positive animals were separated for slaughtering under the supervision of the Egyptian veterinary authorities. Remaining animals were tested every 3 weeks with slaughtering of positive cases and this continued until the remaining animals revealed three successive negative serological

by cross reactions with Although the complement fixation test is the official test for diagnosis of EAE, more sensitive and specific ELISA based tests have been developed. This study aimed to compare three commercial ELISA kits to detect antibodies in ewes and to determine which of the kits had the highest sensitivity. The IDvet kit utilises a MOMP peptide antigen, the MVD-Enfer kit is based

test for Salmonella enterica serotype Typhi (S. Typhi) and Rickettsia infections. Rose Bengal Plate Test (RBPT) and Complement Fixation Test (CFT) were used to screen Brucella infection. Thin and thick blood smears were used to diagnosis malaria. Out of 630 sera screened by DCAT, 83 (13.2%) were reactive to H and/or O antigens for S. Typhi infection. Among these, 46 (55.4%) were reactive