Development of a recombinase polymerase amplification assay with lateral flow dipstick (RPA-LFD) for rapid detection of Shigella spp. and enteroinvasiveEscherichiacoli. Shigella spp. and enteroinvasiveEscherichiacoli (EIEC) are widely distributed and can cause serious food-borne diseases for humans such as dysentery. Therefore, an efficient detection platform is needed to detect Shigella
Imported spring onions related to the first recorded outbreak of enteroinvasiveEscherichiacoli in Denmark, November to December 2021. Between November and December 2021, the first ever recorded outbreak of enteroinvasive in Denmark occurred at national scale. We describe the investigation of this outbreak, which was initially recognised in early December 2021. A total of 88 cases (58 female
L. pseudomesenteroides and L. johnsonii isolated from yaks in Tibet modulate gut microbiota in mice to ameliorate enteroinvasiveEscherichiacoli-induced diarrhea. EnteroinvasiveEscherichiacoli (EIEC) are well-known food-borne pathogens that cause animal intestinal diseases. Lactobacillus is believed to inhibit intestinal pathogens and maintain a healthy gut microbiota. This study aimed
Phylogenetic comparison of enteroinvasiveEscherichiacoli isolated from cases of diarrhoeal disease in England, 2005-2016. We compared the genomes of 60 isolates of enteroinvasive (EIEC) in order to better understand the step-wise evolutionary process from non-pathogenic to host-adapted pathogenic . All isolates belonged to either sequence type (ST) 6, ST99 or ST270. Each ST was located
Novel multiplex PCR assay for identification and subtyping of enteroinvasiveEscherichiacoli and differentiation from Shigella based on target genes selected by comparative genomics. Both Shigella and enteroinvasiveEscherichiacoli (EIEC) can cause enterocolitis, but they have a distinct epidemiology and public health relevance. Current culture-independent testing (CIT) methods to identify
Evaluation of a Culture-Dependent Algorithm and a Molecular Algorithm for Identification of Shigella spp., Escherichia coli, and EnteroinvasiveE. coli Identification of spp., , and enteroinvasive (EIEC) is challenging because of their close relatedness. Distinction is vital, as infections with spp. are under surveillance of health authorities, in contrast to EIEC infections. In this study
Prevalence of multi drug resistant enteropathogenic and enteroinvasiveEscherichiacoli isolated from children with and without diarrhea in Northeast Indian population Diarrheagenic Escherichia coli are associated with infantile diarrhea in the developing countries. The present study was conducted to determine the occurrence and antimicrobial resistance pattern of enteropathogenic and enteroinvasiveE. coli associated with diarrhoea among the paediatric patients. A total of 262 stool samples were collected from children with and without diarrhea from Mizoram, Northeast India. E. coli were isolated and subjected to multiplex PCR to detect virulent genes of EPEC (eaeA and bfpA) and EIEC (ial). Isolates were subjected to antimicrobial sensitivity assay using disc diffusion method. Selected
Probiotic Bacteria and their Supernatants Protect Enterocyte Cell Lines from EnteroinvasiveEscherichiacoli (EIEC) Invasion Probiotic microorganisms have attracted a growing interest for prevention and therapy of gastrointestinal disorders. Many probiotic strains have been shown to inhibit growth and metabolic activity of enteropathogenic bacteria as well as their adhesion and invasion
The Intriguing Evolutionary Journey of EnteroinvasiveE. coli (EIEC) toward Pathogenicity Among the intestinal pathogenic , enteroinvasive (EIEC) are a group of intracellular pathogens able to enter epithelial cells of colon, multiplicate within them, and move between adjacent cells with a mechanism similar to , the ethiological agent of bacillary dysentery. Despite EIEC belong to the same
Uropathogenic Escherichia coli pathogenicity islands and other ExPEC virulence genes may contribute to the genome variability of enteroinvasiveE. coliEnteroinvasiveEscherichiacoli (EIEC) may be the causative agent of part of those million cases of diarrhea illness reported worldwide every year and attributable to Shigella. That is because both enteropathogens have many common characteristics
Two Linked EnteroinvasiveEscherichiacoli Outbreaks, Nottingham, UK, June 2014. EnteroinvasiveEscherichiacoli (EIEC) outbreaks are uncommon in Europe. In June 2014, two EIEC outbreaks occurred in Nottingham, UK, within 2 days; outbreak A was linked to a takeaway restaurant and outbreak B to a wedding party. We conducted 2 analytical studies: a case-control study for outbreak A and a cohort
Phylogenetic background of enterotoxigenic and enteroinvasiveEscherichiacoli from patients with diarrhea in Sirjan, Iran Diarrheagenic (DEC) strains are a major cause of intestinal syndromes in the developing countries. The aim of this study was to determine the prevalence of enterotoxigenic (ETEC) and enteroinvasive (EIEC) in relation to phylogenetic background from patients with diarrhea
Virulence Gene Profile and Multilocus Variable-Number Tandem-Repeat Analysis (MLVA) of EnteroinvasiveEscherichiacoli (EIEC) Isolates From Patients With Diarrhea in Kerman, Iran EnteroinvasiveEscherichiacoli (EIEC) isolates cause dysentery in humans. Several virulence factors associated with EIEC pathogenesis have been characterized. Multilocus variable-number tandem-repeat analysis (MLVA
Investigating the Relatedness of EnteroinvasiveEscherichiacoli to Other E. coli and Shigella Isolates by Using Comparative Genomics EnteroinvasiveEscherichiacoli (EIEC) is a unique pathovar that has a pathogenic mechanism nearly indistinguishable from that of Shigella species. In contrast to isolates of the four Shigella species, which are widespread and can be frequent causes of human
Phylogenetic Analyses of Shigella and EnteroinvasiveEscherichiacoli for the Identification of Molecular Epidemiological Markers: Whole-Genome Comparative Analysis Does Not Support Distinct Genera Designation As a leading cause of bacterial dysentery, Shigella represents a significant threat to public health and food safety. Related, but often overlooked, enteroinvasiveEscherichiacoli (EIEC
Molecular Differentiation of Shigella Spp. from EnteroinvasiveE. Coli A real-time polymerase chain reaction (PCR) assay, amplifying the genes encoding lactose permease and invasion plasmid antigen H , was run on 121 isolates phenotypically classified as spp., enteroinvasive (EIEC), or EIEC O nontypable (ONT). The results were compared with data from a generic multiple-locus variable-number
Draft Genome Sequences of the EnteroinvasiveEscherichiacoli Strains M4163 and 4608-58 We report here the draft genome sequences of enteroinvasiveEscherichiacoli (EIEC) O124:H30 strain M4163 isolated from imported French cheese and EIEC O143:H26 strain 4608-58. The assembled data determined that both strains contain multiple copies of the ipaH gene, as well as the pINV A form of the invasion
Relationship among Shigella spp. and enteroinvasiveEscherichiacoli (EIEC) and their differentiation Shigellosis produces inflammatory reactions and ulceration on the intestinal epithelium followed by bloody or mucoid diarrhea. It is caused by enteroinvasiveE. coli (EIEC) as well as any species of the genus Shigella, namely, S. dysenteriae, S. flexneri, S. boydii, and S. sonnei. This current
Characterisation of an emergent clone of EnteroinvasiveEscherichiacoli circulating in Europe. EnteroinvasiveEscherichiacoli (EIEC) cause intestinal illness indistinguishable from that caused by Shigella, mainly in developing countries. Recently an upsurge of cases of EIEC infections has been observed in Europe, with two large outbreaks occurring in Italy and in the United Kingdom. We have