"Iopanoic acid"

Identification of Iopanoic Acid as Substrate of Type 1 Deiodinase by a Novel Nonradioactive Iodide-Release Assay. Enzymatic 5'- and 5-deiodination are key reactions for local and systemic activation and inactivation of iodothyronines and thyronamines. Expression of the three deiodinase (DIO) isoenzymes is regulated by a number of parameters, including thyroid status, genotype, micronutrient samples from animal experiments (hepatic Dio1 activity in hypo- and hyperthyroid mice) and established DIO inhibitors. As a new but not unexpected finding, the alleged inhibitor iopanoic acid turned out to be a DIO substrate. This finding was confirmed by liquid chromatography-tandem mass spectrometry, and its potential clinical impact requires further studies.

/R) in the presence or absence of exogenous T3 alone or along with 1-850 (inhibitor of T3 receptor) and iopanoic acid (IOA, blocker of T3 release), respectively. A microarray analysis showed that 10,226 gene expression changes in expression across all experimental groups, providing a comprehensive understanding of the cardiac transcriptome. Analysis of 11 candidate genes was conducted using qRT-PCR

analgesics, or potassium iodide or iopanoic acid to reduce the conversion of T4 to the more biologically active form of thyroid hormone, T3.DefinitionSubacute granulomatous thyroiditis is a self-limited inflammation of the thyroid gland. It is associated with a triphasic clinical course that lasts for a few weeks to many months, characterised by transient thyrotoxicosis, hypothyroidism, and then a return

both circulations at different time points. Variables included addition of iopanoic acid (IOP) to block activity of the deiodinase type 3 (D3) and bovine serum albumin (BSA) to trap released T4. T4 and 3,3',5'-triiodothyronine (rT3) concentrations in the perfusates were measured by radioimmunoassays. Results Maternal-to-fetal transfer was slow, with T4 barely detectable in the fetal circulation

to aquatic ecological risk assessment. Until now, very little data on the effects of TH disruption on inflation of the anterior chamber (AC) of the swim bladder were available. The present study used zebrafish exposure experiments with three model compounds with distinct thyroperoxidase and deiodinase inhibition potencies (methimazole, iopanoic acid, and propylthiouracil) to evaluate this linkage. Exposure

% of the patients treated with prednisone alone [45]. In a prospective randomized study of 12 patients, prednisone treatment alone was more effective in rapidly restoring euthyroidism compared to treatment with iopanoic acid [46]. An earlier study had proposed the use of lithium to normalize thyroid function in AIT patients, but the effectiveness is limited and has not been confirmed [47]. Thus, based 2009; 94: 3757–3762. 91. 92. Bogazzi F, Bartalena L, Cosci C, Brogioni S, Dell’Unto E, Grasso L, Aghini-Lombardi F, Rossi G, Pinchera A, Braverman LE, Martino E: Treatment of type II amiodarone-induced thyrotoxicosis by either iopanoic acid or glucocorticoids: a prospective, randomized study. J Clin Endocrinol Metab 2003; 88: 1999–2002. 93. 94. Dickstein G, Shechner C, Adawi F, Kaplan J

, et al. Treatment of hyperthyroidism associated with thyrotropin-secreting pituitary adenomas with iopanoic acid. J Clin Endocrinol Metab. 2004. 89:708-11. [QxMD MEDLINE Link]. [Full Text]. 25. Duncan E, Wass JA. Investigation protocol: acromegaly and its investigation. Clin Endocrinol (Oxf). 1999 Mar. 50(3):285-93. [QxMD MEDLINE Link]. 26. Eugster EA, Pescovitz OH. Gigantism. J

, which was prevented by pretreatment with 1 mg (100 g body weight)(-1) propranolol or 6 mg (100 g body weight)(-1) iopanoic acid (5.9- vs. 2.8-fold; P < 0.05), which blocks D2 activity . In the SKM-D2KO mice, acute treadmill exercise failed to induce PGC-1a fully in soleus muscle (1.9- vs. 2.8-fold; P < 0.05), and in primary SKM-D2KO myocytes there was only a limited PGC-1a response to 1 μm forskolin

expression of human DIO3 specifically in cones, increased cone density by 30-40% in a Rpe65 mouse model of Lebers congenital amaurosis (LCA) and in a Cpfl1 mouse with Pde6c defect model of achromatopsia, compared with their respective untreated controls. Intravitreal and topical delivery of the DIO2 inhibitor iopanoic acid also significantly improved cone survival in the LCA model mice. Moreover

). Consequently, these enzymes are targets of interest for the development of pharmacological compounds with modulatory activities. Until now, the portfolio of inhibitors for these enzymes is limited. In the clinics, the DIO1-specific inhibitor propylthiouracil is in use for treatment of severe hyperthyroidism. Other well-known inhibitors (e.g., iopanoic acid or aurothioglucose) are nonselective and block all Dio1 to human DIO1, DIO2, and DIO3. As proof-of-concept, deiodination reactions catalyzed by these recombinant enzymes were monitored with various nonradioactive substrates and confirmed by liquid chromatography-tandem mass spectrometry. The contrast agent and known DIO inhibitor iopanoic acid was characterized as readily accepted substrate by DIO2 and Dio3. In a screening approach using established

stimulated by (Bu)2cAMP in HUVECs. The blockade of D2 activity through transfection of small interfering RNA (siRNA) specific to D2 as well as by addition of iopanoic acid, a potent D2 inhibitor, abolished Akt phosphorylation, Rac activation, and cell migration induced by T4 but not by T3. The inhibition of TRα1 expression by the transfection of siRNA for TRα1 canceled Akt phosphorylation, Rac activation

of positional CpG-dinucleotides and the respective DIO2 expression in OA-affected and macroscopically preserved articular cartilage from end-stage OA patients. In a human in vitro chondrogenesis model, we measured the effects when thyroid signalling during culturing was either enhanced (excess T3 or lentiviral induced DIO2 overexpression) or decreased (iopanoic acid). OA-related changes in methylation

occurring after orally administered iopanoic acid. Arch Dermatol. 1987 Mar. 123(3):387-8. [QxMD MEDLINE Link]. 12. Young AL, Grossman ME. Acute iododerma secondary to iodinated contrast media. Br J Dermatol. 2014 Jun. 170(6):1377-9. [QxMD MEDLINE Link]. 13. Wilkin JK, Strobel D. Iododerma occurring during thyroid protection treatment. Cutis. 1985 Oct. 36(4):335-7. [QxMD MEDLINE Link

iopanoic acid. Arch Dermatol. 1987 Mar. 123(3):387-8. [QxMD MEDLINE Link]. 12. Young AL, Grossman ME. Acute iododerma secondary to iodinated contrast media. Br J Dermatol. 2014 Jun. 170(6):1377-9. [QxMD MEDLINE Link]. 13. Wilkin JK, Strobel D. Iododerma occurring during thyroid protection treatment. Cutis. 1985 Oct. 36(4):335-7. [QxMD MEDLINE Link]. 14. Paul AK, Al-Nahhas

occurring after orally administered iopanoic acid. Arch Dermatol. 1987 Mar. 123(3):387-8. [QxMD MEDLINE Link]. 12. Young AL, Grossman ME. Acute iododerma secondary to iodinated contrast media. Br J Dermatol. 2014 Jun. 170(6):1377-9. [QxMD MEDLINE Link]. 13. Wilkin JK, Strobel D. Iododerma occurring during thyroid protection treatment. Cutis. 1985 Oct. 36(4):335-7. [QxMD MEDLINE Link

iopanoic acid. Arch Dermatol. 1987 Mar. 123(3):387-8. [QxMD MEDLINE Link]. 12. Young AL, Grossman ME. Acute iododerma secondary to iodinated contrast media. Br J Dermatol. 2014 Jun. 170(6):1377-9. [QxMD MEDLINE Link]. 13. Wilkin JK, Strobel D. Iododerma occurring during thyroid protection treatment. Cutis. 1985 Oct. 36(4):335-7. [QxMD MEDLINE Link]. 14. Paul AK, Al-Nahhas

, et al. Treatment of hyperthyroidism associated with thyrotropin-secreting pituitary adenomas with iopanoic acid. J Clin Endocrinol Metab. 2004. 89:708-11. [QxMD MEDLINE Link]. [Full Text]. 25. Duncan E, Wass JA. Investigation protocol: acromegaly and its investigation. Clin Endocrinol (Oxf). 1999 Mar. 50(3):285-93. [QxMD MEDLINE Link]. 26. Eugster EA, Pescovitz OH. Gigantism. J

, et al. Treatment of hyperthyroidism associated with thyrotropin-secreting pituitary adenomas with iopanoic acid. J Clin Endocrinol Metab. 2004. 89:708-11. [QxMD MEDLINE Link]. [Full Text]. 25. Duncan E, Wass JA. Investigation protocol: acromegaly and its investigation. Clin Endocrinol (Oxf). 1999 Mar. 50(3):285-93. [QxMD MEDLINE Link]. 26. Eugster EA, Pescovitz OH. Gigantism. J

, et al. Treatment of hyperthyroidism associated with thyrotropin-secreting pituitary adenomas with iopanoic acid. J Clin Endocrinol Metab. 2004. 89:708-11. [QxMD MEDLINE Link]. [Full Text]. 25. Duncan E, Wass JA. Investigation protocol: acromegaly and its investigation. Clin Endocrinol (Oxf). 1999 Mar. 50(3):285-93. [QxMD MEDLINE Link]. 26. Eugster EA, Pescovitz OH. Gigantism. J

surgeons. [77] Procedures and preparations are as follows: * * Preoperative preparation to render the patient euthyroid is essential in order to prevent thyrotoxic crisis (thyroid storm). The hyperthyroid state can be rapidly corrected using a combination of iopanoic acid, dexamethasone, beta-blockers, and thioamides. [78, 79] * * This can be accomplished with the use of antithyroid